C . The prepared the glass slides were placed under compound microscope with 10 times (10x) and four hundred times (400x) magnifications to observe images for the presence of conidia and setae. The experiment was conducted by following Completely Randomized Design (CRD) with five replicates. Mangifera indica L. (Mango) belongs to the family-Anacardiaceae. However, identification of causal organism is crucial for taking effective control measure since C. gloeosporioides is sensitive to fungicide. It exhibits morphological variations, which makes it difficult to classify11. Therefore, the objective of this study was to verify the morphological variation of C. gloeosporioides for accurate identification and its managements. According to Vithanage et al.28 anthracnose affected lesions of ripe mangoes and De Souza Serra et al.30 leaves were used to characterize the morphological features. 0000004260 00000 n
Similarly, the lowest variability in S7 due to both small conidia and low acervuli production and S6 was the slow grow at p<0.05 level of significance. 1f). The G1 was showed the highest variability in terms of all characters. 0000004509 00000 n
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After 12 days numerous acervuli with masses of spores were produced on inoculated excised mango leaves (Fig. 0000006052 00000 n
S1: Satkhira, S2: Khulna, S3: Jessore, S4: Bagherhat, S5: Jhenaidah, S6: Rajshahi, S7: Chapai, S8: Dinajpur, S9: Rangpur and L: Leaves, F: Fruits, FLC: Flower clusters, T: Twigs, OMA: Oat meal agar, DMRT was represented in small letters. One of the most common diseases of mango is anthracnose caused by Colletotrichum gloeosporioides in the world. Due to variability in pathogenic isolates, expanded active disease cycle is very complicated to manage12. In the study, the highly variable isolates were S2 based on high growth rate with large conidia but number of acervuli production in S8. 0000094824 00000 n
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Acervulus produces mass of conidia. Cultural methods (mycelial growth rate, color, texture, acervuli, conidial size and setae) and microscopic measurements (ocular micrometer and stage micrometer) were used to characterize the isolates. Morphology of Colletotrichum colonies varies within and among groups, depending on culture medium, substrate and temperature, among other factors. Box plots of six parameters showed differences within the isolate types (Table 2, 3, Fig. Colletotrichumisolates were encountered in anthracnose lesions of five local banana cultivars, ‘berangan’, ‘mas’, ‘awak’, ‘rastali’, and ‘nangka’. Colletotrichum enter into the host tissues directly through a penetration peg that emerges from dome-shaped appressoria. The infected plant parts were cut into 5×5 mm18 (Fig. Based on morphological characteristics, 32 isolates were identified as Colletotrichum gloeosporioidesand 6 isolates as C. musae. 0000002590 00000 n
One way analysis of variance was carried out to examine the significant (p<0.05) difference of all the parameters. The MGR was the highest 9.9-10.21 mm day1 in fruits and lowest in leaves; CS was the highest 22.09-27.54 μm in fruits and lowest in twigs; NOA was the highest 2.11-2.57 cm2 in fruits and lowest in flower cluster; MC was the highest 2.12-2.77 in twigs and lowest in leaves; MT was the highest 2.90-3.12 in flower cluster and lowest in fruits; S was the highest 1.69-1.81 in twigs and lowest in leaves. Which are darkly pigmented with melanin17. They followed the order of F